NGS sequencing-based oncology panels


Frontage offers cost-effective examination of oncogenes from tumor tissue, biofluids, or genomic or cfDNA samples using targeted sequencing of oncopanels developed by industry leading manufacturers such as Illumina and Swift Biosciences. Only panels with expert–defined content are utilized in order to identify variants and fusion events with established relevance to cancer genetics. Extraction services are optimized for multiple samples types including biofluids to facilitate examination of circulating genetic variants collected with minimally invasive strategies.

Examining Genetic Variants with Liquid Biopsy and Tumor Samples

Frontage Laboratories provide ultra-deep sequencing of cancer genes as well as cancer-related genes included in oncopanels. The targeted genes in these oncopanels are among the most studied genes in cancer research.

Our approach allows direct analysis of potentially pathogenic genetic variants residing in known oncogenes. We can isolate cell-free DNA (cfDNA) from patient blood samples, and genomic DNA from tissue biopsies for ultra-deep sequencing of targeted oncogenes. The focused nature of oncopanels can reduce costs and increase the speed of genomic studies because only a limited set of genes are interrogated. Additional benefits include advanced detection of low-frequency variants with deeper sequencing options and production of more manageable datasets than traditional whole genome sequencing studies.

Oncogene Analysis Service

A wide choice of oncopanels are available for selection at Frontage for oncogene analysis service (Table 1). Many of the oncopanels accommodate low input DNA (10 ng) and are therefore amenable to cfDNA oncogene analysis. While some oncopanels include sets of genes involved in various tumor types, others are more specific and contain genes involved in specific cancer types e.g. colorectal cancer and lung cancer. The diversity as well as the specificity of the oncopanels available enable selection of the best oncopanel that satisfies your research interests.

Table I. Oncopanels available for analysis using Frontage’s oncogene analysis service.

Panel Genes Cancer Type Manufacturer FFPE cfDNA oncogene analysis
TrueSight Cancer Sequencing 94 Pan-Cancer Illumina
TruSight Tumor 15 15 Solid Tumor Illumina Yes
TruSight Myeloid Sequencing Up to 54 Hematological Illumina
AmpliSeq – Cancer Hotspot Panel v2 48 Pan-Cancer Illumina Yes
TruSight Tumor 170 170 Solid Tumor Illumina Yes
AmpliSeq – Custom DNA Panel Up to 12,000 amplicons Pan-Cancer Illumina Yes
TruSight Oncology 500 523 Pan-Cancer Illumina Yes Yes
Accel-Amplicon 56G Oncology Panel v2 56 Multiple Swift Biosciences Yes Yes
Accel-Amplicon Plus 57G Pan-Cancer Profiling Panel 57 Pan-Cancer Swift Biosciences Yes
Accel-Amplicon BRCA1 and BRCA2 2 Breast and Ovarian Cancer Swift Biosciences Yes Yes
Accel-Amplicon BRCA1, BRCA2, and PALB2 3 Breast and Ovarian Cancer Swift Biosciences Yes Yes
Accel-Amplicon Comprehensive TP53 1 Multiple Swift Biosciences Yes Yes
Accel-Amplicon EGFR Pathway 4 Multiple Swift Biosciences Yes Yes
Accel-Amplicon™ Plus Colorectal (CRC) Cancer Panel 16 Colorectal Cancer Swift Biosciences Yes Yes
Accel-Amplicon™ Plus Lung Cancer Panel 17 Lung Cancer Swift Biosciences Yes Yes
Accel-Amplicon™ Plus Myeloid Panel 23 Hematological Swift Biosciences Yes Yes
xGen®Acute Myeloid Leukemia Cancer Panel >260 Acute Myeloid Leukemia IDT
xGen®Pan-Cancer Panel 127 Pan-Cancer IDT
Human Breast Cancer Panel 93 Breast Cancer Qiagen Yes
Human Colorectal Cancer Panel 71 Colorectal Cancer Qiagen Yes
Human Lung Cancer Panel 72 Lung Cancer Qiagen Yes
Human Myeloid Neoplasms Panel 141 Hematological Qiagen Yes
Human Actionable Solid Tumor Panel 23 Solid Tumor Qiagen Yes
Human BRCA1 and BRCA2 Panel 2 Breast and Ovarian Cancer Qiagen Yes
Human BRCA1 and BRCA2 Plus Panel 6 Breast and Ovarian Cancer Qiagen Yes
Human Comprehensive Cancer Panel 160 Multiple Qiagen Yes
SeqCap EZ Comprehensive Cancer Design 578 Multiple Roche
SeqCap EZ Human Oncology 981 Multiple Roche
CleanPlex® OncoZoom Panel 65 Multiple Paragon Genomics Yes Yes


 Common Oncology Areas Utilizing Oncopanels

  • Breast cancer
  • Melanoma
  • Colorectal cancer
  • Pancreatic cancer
  • Ovarian cancer
  • Prostate cancer
  • Non-small cell lung cancer
  • Renal cell carcinoma

Sample Types and Sample Submission Instructions

  • Tumor biopsies
  • Genomic DNA (gDNA)
  • Cell-free DNA (cfDNA)
Sample Type Minimum Input Requirements Sample Submission Checklist
Tumor tissue 10 mg Tissue
Genomic or cell-free DNA 10 mg gDNA / cfDNA
Biofluid 4 ml Biofluid